Fig. 2.

In vitro functional analysis of PODXL using PODXL-KO OSCC lines. HSC-2, HSC-2/PODXL-KO #1, HSC-2/PODXL-KO #2, and HSC-2/PODXL-KO #3 cell lines were plated (1500, 3000, and 6000 cells/100 μL/well) in quintuple wells in 96-well plates and were incubated for 48 h. After adding 10 μL of CCK-8 to each well, plates were incubated for 4 h at 37 °C. The values are mean ± SEM. The absorbance was recorded at 450 nm (*P < 0.05, **P < 0.01, Tukey–Kramer's test).