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. Author manuscript; available in PMC: 2019 Jan 2.
Published in final edited form as: Nat Med. 2018 Jul 2;24(8):1204–1215. doi: 10.1038/s41591-018-0086-7

Figure 2. DIPGs infiltrate the brain through branching evolution and genotypic convergence.

Figure 2

(A) Multi-region sampling. Thirteen different tumour-harbouring regions of HSJD-DIPG-010 were sampled post-mortem, from within and outside the pons. Scale bar = 100μm. (B) Exome sequencing was carried out for all regions, with CCFs plotted as a heatmap for all variants found in at least one specimen, with anatomical location highlighted and colour-coded. (C) Phylogenetic trees were reconstructed using neighbour-joining algorithms based upon the nested subpopulation phylogenies calculated as part of EXPANDS, with clearly evident laterally-directed evolution and early escape from the pons of tumour cells found in distinct anatomical sites. (D-F) Eight different tumour-harbouring regions of HSJD-DIPG-014 subjected to the same analysis. (G-I) Eight different tumour-harbouring regions of HSJD-DIPG-015 subjected to the same analysis. Scale bar = 100μm.m