Fig. 2.

S1P promotes fHASC proliferation and viability. A) Evaluation of fHASC proliferation in response to different S1P concentrations. fHASCs were cultured in selected stem cell media, supplemented with delipidated serum, with or without different concentrations of S1P for 24 h, then harvested and incubated with 10 μM EdU solution for 12 h. Percentage of BrDu positive cells was evaluated by flow cytometry. Data are means ± S.D. of three independent experiments. **P < 0.01, ANOVA and Bonferroni multiple comparison test. B) Evaluation of cell growth using the MTT Assay. fHASCs were cultured as in a, the percent survival values for each sample were calculated relative to the vehicle-treated controls (taken as the 100% value). Data are means ± S.D. from three independent experiments, each performed in triplicate. * * P < 0.01, ANOVA followed by Bonferroni multiple comparison test.