Fig. 3.

S1P decreases the expression of specific pluripotent transcription factors in fHASCs. fHASCs were cultured in selected stem-cell media, supplemented with delipidated serum in the presence or in the absence of three different S1P concentrations for 24 h. A) Analysis of expression of pluripotent markers by real-time PCR in fHASCs. Data (mean ± S.D of three experiments) are presented as normalized transcript expression in the samples relative to normalized transcript expression in control cultures (i.e., in basal medium), in which fold change = 1 (dotted line). *P < 0.1, **P < 0.01, ANOVA followed by Bonferroni's correction. B) Analysis of percent expression of selected pluripotent markers by FACS in fHASCs. Data are means ± S.D. of three independent experiments. * P < 0.1, **P < 0.01, ANOVA and Bonferroni multiple comparison test.