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. Author manuscript; available in PMC: 2018 Aug 10.
Published in final edited form as: J Struct Biol. 2018 Feb 24;203(1):37–45. doi: 10.1016/j.jsb.2018.02.006

Fig. 4.

Fig. 4.

Reconstruction using crowdsourced, template-picked, and ‘normalized’ template-picked particle sets. (A) Micrographs from 26S are run through template and crowdsourcing particle picking procedures. (B) Particles are then extracted and run through 2D template-free class averaging. (C) Top 8 2D classes from each particle set. Classes chosen to continue through processing marked in red. (D) All 3D classes from 3D classification of particles chosen in step (C), with similar marking. (E) Final refinements for each particle set.