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. Author manuscript; available in PMC: 2018 Aug 10.
Published in final edited form as: J Mater Chem B. 2017 May 11;5(22):4198–4206. doi: 10.1039/c7tb00657h

Figure 7.

Figure 7

Osteoblast mineralization on nHA/PEUR nanocomposites. (A) Alizarin red staining of human mesenchymal stem cells (hMSCs) cultured on nHA-LTI, nHA and LTI-PEUR. Substrates were stained on day 7. Mineralization (red staining) was observed as early as day 7 on nHA and nHA-LTI nanocomposites but not on LTI-PEUR controls. (B–C) Quantification of alizarin red staining (day 7) by (B) extraction of the dye and (C) area % stained using ImageJ showed that mineralization was most extensive on nHA-LTI nanocomposites. (D) SEM images of the substrates after 7 days of culture with hMSCs. Mineralized nodules (yellow arrows) were observed on nHA-LTI and nHA but not on LTI-PEUR.