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Diamide activates VEGFR2 and potentiates VEGF-A mediated receptor activation. (A) Western blot analysis for VEGFR2 phosphorylation at Y1175 after 15 min of diamide treatment followed by 15 min of VEGF-A treatment. [* = p < 0.0001 compared to control respective control]. (B) CM-H2DCFDA was used to measure the total cellular ROS production after subjecting HAECs to diamide treatment for 30 min [* = p < 0.0001 compared to control].
