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. 2018 Jul 25;19:22–27. doi: 10.1016/j.redox.2018.07.015

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This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 3 — Hydrogen peroxide does not activate VEGFR2. (A and B) Western blot analysis for VEGFR2 phosphorylation at Y1175 after 15 min of H₂O₂ treatment followed 15 min of VEGF-A treatment. [* = p < 0.05 compared to control] (C) CM-H₂DCFDA was used to measure the total cellular ROS production after subjecting HAECs to H₂O₂ for 30 min [* = p < 0.0001 compared to control]. (D) Intracellular concentrations of GSH and GSSG were measured using HPLC following 30 min of H₂O₂ treatment [* = p < 0.05 compared to respective control].