Fig. 4.

Diamide does not stimulate VEGF release and inhibiting PTPs is insufficient to stimulate VEGFR2 activation. (A) VEGF-A was measured using ELISA following 30 min of diamide treatment. (B) Western blot analysis following 30 min of sodium orthovanadate (Na₃VO₄) treatment (to inhibit PTP activity). (C) VEGFR2 phosphorylation at Y1175 following 30 min of 2-AAPA treatment (to inhibit GR activity) followed by 30 min of H₂O₂ treatment. [* = p < 0.05 compared to 2-AAPA] (D) VEGFR2 phosphorylation at Y1175 following 16 h pretreatment with peg-catalase followed by diamide treatment for 30 min. [* = p < 0.05 0 μM diamide compared to 375 μM diamide].