Fig 2. In vivo OS treatment inhibition of the of P. lunatus plasma membrane H+-ATPase activity.
Apices of P. lunatus leaves were treated with either OS or FC and the vanadate-sensitive ATPase phosphohydrolitic activity was assayed as described in Materials and Methods. A. ATPase activity of whole leaf plasma membranes purified from controls and OS-treated plants without (-FC) and with the use of fusicoccin (+FC). B, ATPase activity of proximal half leaf plasma membranes purified from controls and OS-treated plants without (-FC) and with the use of fusicoccin (+FC). C, ATPase activity of plasma membranes purified from the distal half of the leaf plasma membranes purified from controls and OS-treated plants without (-FC) and with the use of fusicoccin (+FC). Data given are the mean of four independent experiments. Metric bars indicate standard deviation. Asterisk indicates significant (P< 0.05) differences.