Fig 8. Attenuation of CHIKV 37997-mCherry in wild-type mice.

Three weeks old C57BL/6 mice were injected with 10³ PFU of indicated viruses at left rear footpad. (A) Rear footpad sizes (width x height) were measured at indicated time points, (B) virus titers in blood were quantified by qRT-PCR at 3 days post-infection, and (C) virus titers in rear ankles were quantified by qRT-PCR at 28 days post-infection. The dashed lines indicate the detection limits. Statistical significance was determined by ANOVA with a Turkey’s multiple comparisons test (*P<0.0332; **P<0.0021; ***p<0.0002, ****p<0.0001) (n = 5–7).