Fig. 8.

Far-western blot of six GST-BRs against salivary and platelet glycoproteins. Lanes contain a lysate of fresh, washed platelets from a single donor (P), or saliva from five different donors (S1 through S5). Glycoproteins were separated by electrophoresis through a 3–8% polyacrylamide gradient, and then stained (top panel) or transferred to nitrocellulose and then probed with GST-BRs as indicated. No signals were detected outside of the cropped region, aside from a high MW band in the platelet sample. The 140–150 kDa BR-reactive bands were previously determined to contain predominantly GPIbα or MG2/MUC7 (Takamatsu et al. 2005, 2006). The precise amount of GPIbα and MG2/MUC7 within the samples is undetermined, but remained constant across the different BRs tested.