Skip to main content
. 2016 Apr 19;25(12):2498–2513. doi: 10.1093/hmg/ddw114

Figure 8.

Figure 8.

The expression of DAPK1, APP phosphorylation at Thr668, and Aβ in the AD patient and normal brains. (A, B) Hippocampal tissues of 12 AD patients and 12 age-matched normal controls were harvested. (A) Proteins from normal and AD hippocampus were used for immunoblotting with anti-DAPK1, anti-pThr668-APP, anti-Aβ or anti-actin antibody. The blots are representative of three independent experiments. (B) Linear regression analysis was used to estimate the correlation between DAPK1 expression and APP phosphorylation at Thr668 (r = 0.7508; Pearson's correlation coefficient). (C) Immunohistochemistry using a monoclonal antibody specific for DAPK1 or a polyclonal antibody specific for phosphorylated APP at Thr668 was conducted on hippocampal regions from AD patient and normal control. ×100 (main photographs); ×400 (insets); scale bar = 50 μm. Representative images from five experiments are shown. (D) Quantitation of DAPK1 and pThr668 APP. Results shown are mean ± standard error (*P < 0.05; ANOVA/Dunnett’s test).