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. Author manuscript; available in PMC: 2019 Jul 17.
Published in final edited form as: Cell Rep. 2018 Jul 17;24(3):538–545. doi: 10.1016/j.celrep.2018.06.061

Figure 3. More RAD51 is required for fork protection than fork reversal.

Figure 3

(A) Fork protection assays in U2OS cells transfected with seven different RAD51 siRNAs. (B–D) U2OS or RADX overexpressing (OE RADX) cells were transfected with the “J11” siRNA (B and D) or “J12” siRNA (C) to RAD51 at the indicated amounts prior to performing the fork protection assay. P values were derived from Kruskal-Wallis ANOVA with a Dunn’s post-test. Immunoblots from transfected cells corresponding to the same samples are shown below the graphs. (E) Model illustrating differential RAD51 requirements.