KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Guinea pig anti-Ex | Maitra et al., 2006 | N/A |
| Guinea pig anti-Sqh1P | Zhang and Ward, 2011 | N/A |
| Mouse anti Sqh | Zhang and Ward, 2011 | N/A |
| Mouse anti-Flag | Sigma | Cat#F1804 |
| Rat anti-Ecad | Developmental Studies Hybridoma Bank | Cat#AB_528120 |
| Guinea pig anti-Sd | Guss et al., 2013 | N/A |
| Rabbit anti-Yki | Oh and Irvine, 2008 | N/A |
| Rabbit anti-HA | Santa Cruz | Cat#sc-805 |
| Mouse anti-Flag agarose beads | Sigma | Cat#A2220 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Normal goat serum | Jackson ImmunoResearch | Cat# 005000001 |
| 16% formaldehyde | Electron Microscopy Sciences (EMS) | Cat#15710 |
| S2 medium | Sigma | Cat# S9895 |
| 10% Fetal Bovine Serum | Thermo Fisher Scientific | Cat#16000044 |
| Didodecyldimethylammonium bromide | Sigma | Cat# 359025 |
| Methyl salicylate | Thermo Fisher Scientific | Cat#119368 |
| Experimental Models: Organisms/Strains | ||
| D. melanogaster: UAS-sqhDD | Mitonaka et al., 2007 | N/A |
| D. melanogaster: UAS-Flag-Yki | This study | N/A |
| D. melanogaster: UAS-Myr-Flag-Yki (Random insertion) | This study | N/A |
| D. melanogaster: UAS-Myr-Flag-Yki (Site specific integration) | This study | N/A |
| D. melanogaster: UAS-Myr-Flag-YkiΔNH | This study | N/A |
| D. melanogaster: UAS-Myr-Flag-Yki1-34-GFP(Site specific integration) | This study | N/A |
| D. melanogaster: UAS-Myr-Flag-YkiAcidA (Site specific integration) | This study | N/A |
| D. melanogaster: UAS-yki RNAi | Vienna Drosophila RNAi Center | 40497 |
| D. melanogaster: UAS-yki RNAi | Vienna Drosophila RNAi Center | 104523 |
| D. melanogaster: UAS-sd RNAi | Vienna Drosophila RNAi Center | 101497 |
| D. melanogaster: UAS-Dcr2 | Bloomington Drosophila Stock Center | 24650 |
| D. melanogaster: UAS-Dcr2 | Bloomington Drosophila Stock Center | 24651 |
| D. melanogaster: UAS-Strn-Mlck RNAi | Bloomington Drosophila Stock Center | JF02278 |
| D. melanogaster: UAS-Strn-Mlck RNAi | Bloomington Drosophila Stock Center | JF02170 |
| D. melanogaster: UAS-Strn-Mlck RNAi | Bloomington Drosophila Stock Center | HMS01665 |
| D. melanogaster: UAS-Strn-Mlck RNAi | Bloomington Drosophila Stock Center | HMS02663 |
| D. melanogaster: UAS-Rok RNAi | Vienna Drosophila RNAi Center | 104675 |
| D. melanogaster: UAS-Rok RNAi | Bloomington Drosophila Stock Center | HMS01311 |
| D. melanogaster: UAS-Drak RNAi | Bloomington Drosophila Stock Center | JF03385 |
| D. melanogaster: UAS-Drak RNAi | Bloomington Drosophila Stock Center | GL00087 |
| D. melanogaster: UAS-Drak RNAi | Bloomington Drosophila Stock Center | HMS02822 |
| D. melanogaster: UAS-sqa RNAi | Bloomington Drosophila Stock Center | JF02277 |
| D. melanogaster: UAS-sqa RNAi | Vienna Drosophila RNAi Center | 101640 |
| D. melanogaster: UAS-sqa RNAi | Vienna Drosophila RNAi Center | 107241 |
| D. melanogaster: UAS-bt RNAi | Bloomington Drosophila Stock Center | JF01107 |
| D. melanogaster: UAS-bt RNAi | Bloomington Drosophila Stock Center | JF01108 |
| D. melanogaster: UAS-gek RNAi | Bloomington Drosophila Stock Center | GL00299 |
| D. melanogaster: UAS-gek RNAi | Bloomington Drosophila Stock Center | HMS04488 |
| D. melanogaster: UAS-sti RNAi | Bloomington Drosophila Stock Center | GL00312 |
| D. melanogaster: UAS-sti RNAi | Bloomington Drosophila Stock Center | HMC03586 |
| D. melanogaster: UAS-sti RNAi | Bloomington Drosophila Stock Center | HMJ30325 |
| D. melanogaster: UAS-AMPKα RNAi | Bloomington Drosophila Stock Center | JF01951 |
| D. melanogaster: UAS-AMPKα RNAi | Bloomington Drosophila Stock Center | HMS00362 |
| D. melanogaster: UAS-AMPKα RNAi | Bloomington Drosophila Stock Center | GL00004 |
| D. melanogaster: UAS-AMPKα RNAi | Bloomington Drosophila Stock Center | HMC04979 |
| D. melanogaster: UAS-AMPKα RNAi | Vienna Drosophila RNAi Center | 1827 |
| D. melanogaster: UAS-AMPKα RNAi | Vienna Drosophila RNAi Center | 106200 |
| D. melanogaster: 82BFRT wtsX1 | Xu et al., 1995 | N/A |
| D. melanogaster:42DFRT ykiB5 | Huang et al., 2005 | N/A |
| D. melanogaster:19AFRT sd47M | Wu et al., 2008 | N/A |
| D. melanogaster: sqhAX3/FM | Jordan and Karess, 1997 | N/A |
| D. melanogaster: Diap1-lacZ | Hay et al., 1995 | N/A |
| D. melanogaster: ex-lacZ | Hamaratoglu et al., 2006 | N/A |
| D. melanogaster: fj-lacZ | Bloomington Drosophila Stock Center | 6370 |
| D. melanogaster: sqh:mCherry | Martin et al., 2009 | N/A |
| D. melanogaster: sqh:GFP | Vienna Drosophila RNAi Center | 318484 |
| D. melanogaster: zip:GFP | Rauskolb et al., 2014 | N/A |
| D. melanogaster: zip1/Cyo | Young et al., 1993 | N/A |
| D. melanogaster: w1118; ykiB5 {yki-YFP} VK37 | This study | N/A |
| D. melanogaster: nub-Gal4 | Bloomington Drosophila stock center | 38418 |
| D. melanogaster: hh-Gal4 | Tetsuya Tabata | N/A |
| D. melanogaster: ap-Gal4 | Bloomington Drosophila stock center | 3041 |
| D. melanogaster: w1118; {Wts-YFP} VK37 | This study | N/A |
| D. melanogaster: y M{vas-int.Dm}ZH-2A w; M{3xP3-RFP.attP}ZH-86Fb | Bloomington Drosophila stock center | 24749 |
| D. melanogaster: UAS-Strn-Mlck-HA | This study | N/A |
| D. melanogaster: Kr[If-1]/CyO; UAS-GrabFP-AInt/TM6 | Bloomington Drosophila stock center | 68178 |
| D. melanogaster: y w vas-Cas9 | Bloomington Drosophila stock center | 52669 |
| Oligonucleotides | ||
| Yki 5′ Homology arm
forward 5AGCACCTGCgatcTACTGAAAGTAGAGGTGATTAGTTGTTAACGAG3 |
Integrated DNA Technologies | N/A |
| Yki 5′ Homology arm
reverse 5TTTCTAGGGTTAACATATAAAAGGCACTTATTGTATACAAACGAAAAC3 |
Integrated DNA Technologies | N/A |
| Yki 3′ Homology arm forward including
AcidA
substitution 5TTCTAGGGTTAACGACGATGTCAGCCAGCAGCAATACAAACAGCCTGATCGccAAGGccATCGcCGcCGccGcCATGCTTTCGCCGATtAAGTCgAACAACCTG3 |
Integrated DNA Technologies | N/A |
| Yki 3′ Homology arm
reverse 5CGACGGCTCTTCCTTGTAGTATGACTGCAAGTAGAGAGAACAC3 |
Integrated DNA Technologies | N/A |
| Guide RNA
sequence 5ACCAGGTTGTTGGACTTGAT3 |
Integrated DNA Technologies | N/A |
| Recombinant DNA | ||
| pU6-BbsI-chiRNA | Addgene | Cat#45946 |
| PAWH | Drosophila Genomics Resource Center | 1096 |
| pUAST-Myr | Neisch et al., 2013 | N/A |
| pUASTattB | Bischof et al., 2007 | N/A |
| Strn-Mlck EST | Drosophila Genomics Resource Center | RH61010 |
| pAWH-Strn-Mlck (Strn-Mlck-HA) | This study | N/A |
| pHD-ScarlessDsRed | Drosophila Genomics Resource Center | 1364 |
| Software and Algorithms | ||
| Image J | NIH | https://imagej.nih.gov/ij/ |
| R 3.5.0 | The R Foundation | https://www.r-project.org/ |