Skip to main content
. Author manuscript; available in PMC: 2019 Aug 2.
Published in final edited form as: Cell Stem Cell. 2018 Jul 12;23(2):289–305.e5. doi: 10.1016/j.stem.2018.06.013

Figure 2. Identification and Characterization of Reprogramming Markers.

Figure 2

(A) Transcriptional profiles of candidate cell-surface markers. SSEA-1− (red) and SSEA-1+ intermediates (blue) derived from het/het MEFs were analyzed by RNA-seq. Results are shown as the mean of 3 replicates.

(B) Flow analysis of het/het MEF reprogramming. Representative dot plots are gated on total live cells (red), SSEA-1+ cells (blue), or Oct4-GFP+ cells (green). Vertical lines indicate the cutoff between negative and positive staining based on an isotype-matched negative control.

(C) Quantification of the plots in Figure 2B. Lines show the percentage of total live cells (red), SSEA-1+ cells (blue), and Oct4-GFP+ cells (green) positive for the indicated markers.

(D) Schematic of reprogramming marker expression based on the results above and Figure S2.