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. Author manuscript; available in PMC: 2019 Aug 15.
Published in final edited form as: J Immunol. 2018 Jul 6;201(4):1186–1193. doi: 10.4049/jimmunol.1800052

Figure 3. IV+ CD4+ T cells exhibit superior control of intracellar Ftt replication in vitro.

Figure 3

IV+ and IV− CD4+ T cell populations in immune mice were labeled 28 days after vaccination using intravascular staining. Lungs were processed into single cell suspension and sorted into FITC+ and FITC− populations. CD4+ T cells were enriched from these sorted pools or purified directly from naïve lungs and added to Ftt-infected bone marrow macrophages at a ratio of 1 T cell per 2 macrophages. Forty-eight hours later, the number of intracellular bacteria were enumerated. n=3 biological replicates per group. Data are representative of two independent experiments. Error bars represent (SEM). Statistical significance between naïve, IV− CD4+, and IV+ CD4+ was determined using an ANOVA with Tukey’s post-test; * indicates p ≤ 0.05.