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. Author manuscript; available in PMC: 2019 Jul 5.
Published in final edited form as: Mol Cell. 2018 Jul 5;71(1):155–168.e7. doi: 10.1016/j.molcel.2018.06.016

Figure 5. Sup35 PrD mutants illuminate nucleation mechanisms in vivo.

Figure 5.

(A) DAmFRET plots of select mutants and scrambled sequence variants (of overall identical amino acid composition) of Sup35 PrD in [PIN+] and [pin−] cells. Boxes in the plots for #25 designate the region considered prion-positive, with the percentage of total cells indicated. See also Fig. S5A. The red and black arrows indicate the “intermediate” and “low” AmFRET populations, respectively, that were sorted for microscopy analyses.

(B) Quantification of fluorescence recovery times after half-puncta photobleaching for low (n = 28 cells) and intermediate AmFRET (n = 18 cells) states of [pin−] cells expressing Sup35 PrD #24. Boxes cover the SE, while the square inside the box shows the mean and the line shows the median. Whiskers delineate the 5th and 95th percentiles. See also Fig. S5 C, D.