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. Author manuscript; available in PMC: 2019 Jul 5.
Published in final edited form as: Mol Cell. 2018 Jul 5;71(1):155–168.e7. doi: 10.1016/j.molcel.2018.06.016

Figure 6. Nucleation barriers enable proteotoxic assemblies to accumulate.

Figure 6.

(A) [pin-] cells harboring plasmids encoding the indicated proteins spotted as five-fold serial dilutions onto media with either galactose (inducing) or glucose (repressing).

(B) Budding indices of cells expressing Sup35 PrD #24 or unfused mEos3.1 to the same intensity (5000 – 7000 AU). Solid red and checkered bars denote [pin-] and [PIN+] cells, respectively. The latter is divided into “high” (amyloid) and “low” (non-amyloid) AmFRET subpopulations. Shown are means of five experiments; error bars represent 95 % CI; ****, p<0.0001 (ANOVA).