Figure 1.

Cinnamtannin B-1 (CTB-1) decreases the viability of colon cancer cells. (A) DLD-1 and (B) COLO 201 colon cancer cell lines were treated with increasing concentrations of CTB-1 or the DMSO vehicle control for 24, 48 and 72 h, then assessed for cell viability via MTT assay. The half maximal effective concentration (EC₅₀) of CTB-1 against colon cancer cell lines following treatment for 72 h is displayed in the graph, calculated from the equation of the line-of-best-fit. (C) CCD 841 CoN normal colon epithelial cells were treated with CTB-1 within the effective concentration range to assess cytotoxicity via MTT assay. For all graphs, the mean value (% vehicle control-VT) ± SEM from 3 replicates is shown. Significant differences between time-matched CTB-1-treated and vehicle-treated samples are denoted by (*) for 24 h, (+) for 48 h, and (#) for 72 h (⁺p<0.05, ^##p<0.01, ***p<0.001, ^{****,++++,####}p<0.0001, Dunnett's multiple comparisons test).