Table I.
Antibodies used in flow cytometry.
| Antibody (clone, company, cat. no.) | Isotype (clone, company, cat. no.) |
|---|---|
| FITC-anti-mouse CD45 (B3821F4A/N901/UCHT1, Beckman Coulter, Tokyo, Japan, CO6607071) | FITC-rat IgG, 2b, k isotype (eBRG1, eBioscience, 11-4031-82) |
| FITC-anti-mouse c-mesenchymal-epithelial transition (eBioclone7, eBioscience, Tokyo, Japan, 11-8854-80) | FITC-rat IgG1, k isotype (eBRG1, eBioscience, 11-4301-82) |
| FITC-anti-mouse CD80 (16-10A1, BioLegend, Tokyo, Japan, 104705) | FITC-Arm Hamster, IgG isotype (eBio299Arm, eBioscience, 11-4888-81) |
| FITC-anti-mouse CD86 (GL1, BioLegend, 105005) | FITC-rat IgG, 2b, k isotype (eB149/10H5, eBioscience, 11-4031-82) |
| PE-anti-mouse 4-1BB ligand (TKS-1, BioLegend, 107105) | PE-mouse IgG, 2a, k isotype (MOPC-173, BioLegend, 400211) |
| FITC-anti-mouse CD11b (M1/70, eBioscience, 11-0122-85) | FITC-rat IgG, 2b, k isotype (eBRG1, eBioscience, 11-4031-82) |
| APC-anti-mouse Ly6G (RB6-8C5, eBioscience, 17-5931-82) | APC-Rat, IgG, 2a, k isotype (eBR2a, eBioscience, 17-4321-81) |
| PE-anti-mouse Ly6G (RB6-8C5, eBioscience, 12-5931-82) | PE-mouse IgG, 2a, k isotype (MOPC-173, BioLegend, 40021) |
| APC-anti-mouse Ly6C (HK1.4, BioLegend, 128015) | APC-Rat, IgG, 2c, k isotype (RTK4174, BioLegend, 400713) |
| PE-Cyanine5-anti-mouse F4/80 (BM8, BioLegend, 123111) | PE-Cyanine5-Rat, IgG, 2a, k isotype (eBR2a, eBioscience, 35-4321-80) |
| FITC-anti-mouse CD8a (53-6.7, eBioscience, 11-0081-81) | FITC-Rat, IgG, 2a, k isotype (eBR2a, eBioscience, 11-4321-82) |
| PE-Cyanine5-anti-mouse CD3e (145-2C11, eBioscience, 15-0031-82) | PE-Cyanine5-Arm Hamster, IgG isotype (eBio299Arm, eBioscience, 15-4888-82) |
| APC-anti-mouse CD4 (RM4-5, eBioscience, 17-0042-81) | APC-Rat, IgG, 2a, k isotype (eBR2a, BioLegend, 400512) |
| FITC-anti-mouse CD25 (PC61.5, BD, 558689) | FITC-mouse IgG1, λ isotype (G0114F7, BioLegend, 401913) |
| PE-anti-mouse FoxP3 (NRRF-30, eBioscience, 12-4771-80) | PE-mouse IgG, 2a, k isotype (MOPC-173, BioLegend, 400211) |
FITC, fluorescein isothiocyanate; APC, allophycocyanin; PE, phycoerythrin.