Effect of gallic acid, caffeoyl-hydroxycritric acid, neochlorogenic acid and methanolic and dichloromethane extracts of H. acetosella, H. cannabinus and H. sabdariffa on MPO activity measured by SIEFED.
Notes: The percentage of inhibition was calculated for each sample concentration versus the corresponding control (MPG + DMSO), taken as 100% (mean ± SD. n = 6). Samples vs. Control DM5O:ns: no significance, p-values (****< p0.0001) calculated by two-way ANOVA indicated a significant effect vs. DMSO control set as 100% response. ns = not significant vs. DMSO control. There are statistical differences between dichloromethane and methanolic extracts of all Hibiscus species. Dichloromethane extracts are more active than methanolic extracts, caffeoyl-hydroxycitric and neochlorogenic acids are less active than gallic acid used as positive control. Extract of H. cannabinus are the most active, followed by those of H. acetosella and H. sabdariffa.