Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information | |||
|---|---|---|---|---|---|---|---|
| Gene (Homo sapiens) | LRRC8A | Origene | Uniprot: Q8IWT6 | ||||
| Cell line (Homo sapiens) | Freestyle 293-F | ThermoFisher | RRID: CVCL_D603 | ||||
| Cell line (Homo sapiens) | HeLa | ATCC | RRID: CVCL_0030 | ||||
| Recombinant DNA reagent |
pcDNA3.1/Zeo(-) | ThermoFisher | cat no: V86520 | ||||
| Chemical compound | digitonin | Sigma-Aldrich | CAS Number: 11024-24-1 |
||||
| Software, algorithm | MotionCor2 | doi:10.1038/nmeth.4193 | |||||
| Software, algorithm | EMHP | doi:10.1093/bioinformatics/btx500 | |||||
| Software, algorithm | Gctf | doi:10.1016/j.jsb.2015.11.003 | |||||
| Software, algorithm | FindEM template correlator |
doi:10.1016/j.jsb.2003.11.007 | |||||
| Software, algorithm | cryoSPARC | doi: 10.1038/nmeth.4169 | https://cryosparc.com/ | ||||
| Software, algorithm | RELION | RRID:SCR_016274 | |||||
| Software, algorithm | Rosetta | RRID:SCR_015701 | https://www.rosettacommons.org/software | ||||
| Software, algorithm | Robetta | http://robetta.bakerlab.org/ | |||||
| Software, algorithm | COOT | RRID:SCR_014222 | http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ | ||||
| Software, algorithm | Phenix | RRID:SCR_014224 | https://www.phenix-online.org/ | ||||
| Software, algorithm | PyMOL | PyMOL Molecular Graphics System, Schrodinger LLC |
RRID:SCR_000305 | http://www.pymol.org/ | |||
| Software, algorithm | UCSF Chimera | UCSF | RRID:SCR_004097 | http://plato.cgl.ucsf.edu/chimera/ | |||