Video 1. Crystal structure of RIG-I Δ2CARD C268F and close-up of the active site.

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DOI: 10.7554/eLife.38958.010

The Singleton-Merten syndrome (SMS) mutation F268, as well as K270 and E702, are represented by a stick model. Theoretic locations of ADP·BeF₃ and Mg²⁺ are indicated in faint sticks and spheres, respectively, according to a superposition with RIG-I Δ2CARD in complex with RNA and nucleotide analogue (PDB 5E3H). K270 is located at the Mg²⁺-binding site, whereas E702 occupies the BeF₃ (ATP γ-phosphate) position.