Table 2. EM data collection and refinement statistics of XaxAB.
| Data collection | |
|---|---|
| Microscope | Titan Krios (Cs corrected, XFEG) |
| Voltage (kV) | 300 |
| Camera | Falcon III (counting mode) |
| Magnification | 59 k |
| Pixel size (Å) | 1.11 |
| Number of frames | 180 |
| Total electron dose (e-/Å2) | 44 |
| Exposure time (s) | 60 |
| Defocus range (µm) | 1.0–2.6 |
| Number of particles | 139,286 |
| Atomic Model Composition | |
| Non-Hydrogen atoms | 72,436 |
| Protein Residues | 9139 |
| Refinement (Phenix) | |
| RMSD bond | 0.006 |
| RMSD angle | 0.98 |
| Model to map fit, CC mask | 0.85 |
| Resolution (FSC@0.143, Å) | 4.0 |
| Map sharpening B-Factor (Å2) | −170 |
| Validation | |
| Clashscore, all atoms | 4.68 |
| Poor Rotamers (%) | 0.92 |
| Favored rotamers (%) | 94.56 |
| Ramachandran outliers (%) | 0 |
| Ramachandran favored (%) | 97.42 |
| Molprobity score | 1.35 |