Figure 2. Caudal activation in the proximal tubule is independent of Wingless signalling.

Maximum intensity projections of approximately stage 16 Drosophila embryos, oriented anterior left, stained with anti-Cut to mark the entire tubule and anti-Caudal (Cad). (A) Control (w¹¹¹⁸) embryo in which Caudal can be observed staining the nuclei of the posterior midgut and the proximal halves of the tubules Note Cad expression extends more distally than Odd (compare with Figure 1B). (B) Embryo expressing pan/dTCF-∆N using the cut promoter. (C) Embryo expressing arm^S10 using the cut promoter. An enlargement of the Caudal-expressing domain from an anterior tubule corresponding to boxed region is provided (bottom panel). Scale bar = 50 μm.