(A) Representative photomicrographs of D2 adult animals expressing neuronal Q40∷YFP; hsp-6p∷gfp in WT or egl-20 animals.
(B) Quantification of hsp-6p∷gfp expression. The genotypes are as in (A).
(C) Immunoblots of GFP expression. The genotypes are as in (A).
(D) Representative photomicrographs of D2 adult animals expressing neuronal Q40∷YFP; DVE-1∷GFP in WT and egl-20 animals. Arrows highlight the DVE-1∷GFP signal localized in the intestinal nuclei.
(E) Quantification of the number of intestinal nuclei with DVE-1 puncta. The genotypes are as in (D).
(F) Representative photomicrographs of D1 animals expressing hsp-6p∷gfp in WT or egl-20 animals grown on EV or cco-1 RNAi from hatching.
(G) Quantification of hsp-6p∷gfp expression. The genotypes are as in (F).
(H) Representative photomicrographs of hsp-6p∷gfp expression in neuronal specific cco-1 RNAi animals in WT or egl-20 background.
(I) Quantification of hsp-6p∷gfp expression. The genotypes are as in (H).
*** p< 0.0001, ns denotes p > 0.05 via t-test. Error bars, SEM. n ≥ 15 worms.
See also Figure S3.