(A) Representative photomicrographs of rgef-1p∷egl-20; hsp-6p∷gfp reporter animals in WT, vps-35, bar-1 RNAi, or pop-1 RNAi background.
(B) Quantification of hsp-6p∷gfp expression. The genotypes are as in (A).
(C) Immunoblot of GFP expression. The genotypes are as in (A).
(D) Representative photomicrographs of gly-19p∷egl-20; hsp-6p∷gfp reporter animals in WT, vps-35, bar-1 RNAi, or pop-1 RNAi background.
(E) Quantification of hsp-6p∷gfp expression. The genotypes are as in (D).
(F) Immunoblot of GFP expression. The genotypes are as in (D).
*** p< 0.0001 via t-test. Error bars, SEM. n ≥ 15 worms.
See also Figure S6.