Fig. 6.

Intestinal barrier integrity is compromised in hypertensive mice. (A) Plasma samples were diluted 1/10,000 and added to ELISA sandwich with antibody to haptoglobin. The plate was washed and then exposed to substrate to reveal expression. Absorbance was detected at 480 nm wavelength. Bar graph, n=5/group. *p < 0.05 (B) gDNA extracted from kidney was purified and set up for a PCR reaction, using 100ng sample and primers specific to bacteria. Samples at the end of the PCR run were loaded onto a 1% agarose gel and imaged.