REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
BrdU	Santa Cruz Biotechnology	Cat# sc-32323, RRID:AB_626766
H3K27ac	Abcam	Cat# ab4729, RRID:AB_2118291
PPARα	Santa Cruz Biotechnology	Cat# sc-398394
SREBP1c	Abcam	Cat# ab3259, RRID:AB_303650
RAD21	Cell Signaling Technology	Cat# 4321S, RRID:AB_1904106
Chemicals and Reagents
TRIzol	ThermoFisher Scientific	Cat# 10296010
CircLigase ssDNA Ligase	Epicentre	Cat# CL4111K
BrUPT	Sigma	Cat# B7166
cOmplete, EDT-free Protease Inhibitor Cocktail	Roche	Cat# 11873580001
SUPERase-In RNAse Inhibitor	ThermoFisher Scientific	Cat# AM2696
Deuterium oxide	Sigma	Cat# 435767
WY 14,643	Cayman	Cat# 70730
9,10-3H-palmitoyl-CoA	PerkinElmer	Cat# NET043001MC
Low Range ssRNA Ladder	New England Biolabs	Cat# N0364S
APE 1	New England Biolabs	Cat# M0282S
TrackIt 25 bp DNA Ladder	Thermo Fisher Scientific	Cat# 10488-022
Novex TBE-Urea Gels, 10%, 10 well	Thermo Fisher Scientific	Cat# EC6875BOX
Novex TBE Gels, 10%, 12 well	Thermo Fisher Scientific	Cat# EC62752BOX
Novex TBE Running Buffer (5x)	Thermo Fisher Scientific	Cat# LC6675
ChIP DNA Clean & Concentrator- Capped column	Zymo Research	Cat# D5205
DNA LoBind Microcentrifuge Tube	Eppendorf	Cat# 30108.051
Exonuclease I (E. coli)	New England Biolabs	Cat# M0293L
Novex TBE-Urea Sample Buffer (2x)	Thermo Fisher Scientific	Cat# LC6876
Novex Hi-Density TBE Sample Buffer (5x)	Thermo Fisher Scientific	Cat# LC6678
SuperScript III Reverse Transcriptase	Thermo Fisher Scientific	Cat# 18080-051
Deoxynucleotide (dNTP) Solution Mix	New England Biolabs	Cat# N0447S
SYBR Gold Nucleic Acid Gel Stain	Thermo Fisher Scientific	Cat# S11494
Phusion Hot Start II DNA Polymerase	Thermo Fisher Scientific	Cat# F-549L
E. coli Poly (A) Polymerase	New England Biolabs	Cat# M0276S
UltraPure Glycogen	Thermo Fisher Scientific	Cat# 10814010
TURBO DNA-free Kit	Thermo Fisher Scientific	Cat# AM1907
Micro Bio-Spin P-30 Gel Columns	Bio-Rad	Cat# 732-6250
RNA Fragmentation Reagents	Thermo Fisher Scientific	Cat# AM8740
T4 Polynucleotide Kinase	New England Biolabs	Cat# Y904L
SSPE (20x)	Thermo Fisher Scientific	Cat# AM9767
NuPAGE 4-12% Bis-Tris Protein Gels, 1.0 mm, 10-well	Thermo Fisher Scientific	Cat# NP0321 BOX
NuPAGE MOPS SDS Running Buffer (20X)	Thermo Fisher Scientific	Cat# NP0001
NuPAGE LDS Sample Buffer (4X)	Thermo Fisher Scientific	Cat# NP0008
HistoPrep Media	Thermo Fisher Scientific	Cat# SH75-125D
AG 1-X8 formate resin	Bio-Rad	Cat# 1401443
Critical Commercial Assays
RNeasy Mini Kit	Qiagen	Cat# 74106
High-Capacity cDNA Reverse Transcription Kit	Applied Biosystems	Cat# 4368813
Triglyceride Assay Kit	StanBio	Cat# 2100-430
Deposited Data
GRO-seq in DIO	This study	GEO: GSE108878
H3K27ac ChIP-seq	This study	GEO: GSE108878
GRO-seq in NC	(Fang et al., 2014)	GEO: GSE59486
H3K27ac ChIP-seq	(Koike et al., 2012)	GEO: GSE39860
PPARα ChIP-seq	(Lee et al., 2014)	GEO: GSE61817
Experimental Models: Organisms/Strains
Mouse: C57BL/6J	Jackson labs	000664
Mouse: C57BL/6J DIO	Jackson labs	380050
Mouse: Scap floxed	Timothy Osborne	(Matsuda et al., 2001)
Mouse: Pparγfloxed	Jackson labs	004584
Mouse: Pparα −/−	Jackson labs	008154
Oligonucleotides
See Table S3 for qPCR primer list
See Table S4 for ChIP-qPCR primer list
Software and Algorithms
Bowtie 1.1.1	(Langmead et al., 2009)	http://bowtie-bio.sourceforge.net/index.shtml
SAMtools 1.1	(Li et al., 2009)	http://samtools.sourceforge.net/
Bedtools 2.19	(Quinlan and Hall, 2010)	http://bedtools.readthedocs.org/en/latest/
edgeR	(Robinson et al., 2010)	https://bioconductor.org/
pheatmap		https://cran.r-project.org/web/packages/pheatmap/
HOMER v4.6	(Heinz et al., 2010)	http://homer.salk.edu/homer/
JTK_CYCLE	(Hughes et al., 2010)	https://github.com/mfcovington/jtk-cycle
IMAGE	(Madsen et al., 2017)	https://github.com/JesperGrud/IMAGE
R 3.1.0		www.r-project.org/
Perl v5.16		https://www.perl.org
GATHER	(Chang and Nevins, 2006)	http://changlab.uth.tmc.edu/gather/gather.py
Adobe Illustrator	Adobe	Adobe.com
Other
AAV-TBG-GFP	UPenn Vector Core	N/A
AAV-TBG-Cre	UPenn Vector Core	N/A
Rodent Diet with 60% kcal% fat	Research Diets	Cat# D12492
Normal chow	LabDiet	Cat# 5010