Fig. 4.

Quantitative RT-PCR and western blot analysis of GDF-15 expression in fully grown human oocytes: a amplification curves re-constructed using data exported from LightCycler 480 to SigmaPlot software, presented as an average of all amplified curves obtained for particular sample (from all replicates) and gene (LNCaP cells sorted in set numbers serving as positive and calibration controls); b calculated Cp values (by LightCycler 480 software [absolute quantification 2nd derivative maximum analysis]). $ ~ 5 replicates; § ~ 2 replicates; and c expression of GDF-15 in oocytes (OO, 17 oocytes) analyzed by western blot under reduced condition (LNCaP cells and seminal plasma [SP] samples serving as positive controls)