Table 2.
Embryological data of processed cases
| Case | COC retrieved | Performed ICSI | Oocytes fertilized (day 1 assessment) | Successfully fertilized oocytesa | Fertilization rateb | Embryos biopsied | Blastocyst formation ratec |
|---|---|---|---|---|---|---|---|
| HTT-case | 16 COC (3 GV, 1 MI, 12 MII) | 13 | 1 deg, 2 × 0 PN, 10 × 2 PN | 10 | 0.77 | 7 × 2 PN | 0.70 |
| ACTA2-case | 18 (1 empty ZP, 1 MI, 16 MII) | 17 | 4 deg, 2 × 0 PN, 11 × 2 PN | 11 | 0.65 | 9 × 2 PN | 0.82 |
| TPP1-case | 8 COC (2 GV, 6 MII) | 6 | 1 deg, 1 × 1 PN, 1 × 3 PN, 3 × 2 PN | 4 | 0.67 | 3 × 2 PN | 0.75 |
| ALOX12B-case (two stimulations) | 15 COC (1 empty ZP, 2 atretic, 3 GV, 1 MI, 8 MII) | 9 | 2 deg, 1 × 0 PN, 2 × 3 PN, 3 × 2 PN | 5 | 0.56 | 2 × 2 PN, 1 × 3 PN | 0.6 |
| 16 COC (2 empty ZP, 2 GV, 2 MI, 10 MII) | 12 | 2 deg, 1 × 1 PN, 9 × 2 PN | 9 | 0.75 | 9 × 2 PN | 1.0 | |
| DMD-case 1 | 18 COC (1 empty ZP, 1 MI, 16 MII) | 17 | 4 deg, 2 × 0 PN, 11 × 2 PN | 11 | 0.65 | 9 × 2 PN | 0.82 |
| DMD-case 2 | 22 COC (2 empty ZP, 2 GV, 18 MII) | 18 | 1 × 1 PN, 17 × 2 PN | 17 | 0.94 | 16 × 2 PN, 1 × 1 PN | 1.00 |
| MTM1-case (two stimulations) | 5 COC (1 GV, 4 MII) | 4 | 3 × 0 PN, 1 × 1 PN | 0 | 0.00 | NA | NA |
| 10 COC (2 ZP, 1 MI, 7 MII) | 8 | 2 deg, 4 × 0 PN, 2 × 3 PN | 2 | 0.25 | 0 | 0.00 | |
| GLB1-case | 15 COC (1 ZP, 14 MII) | 14 | 2 × 0 PN, 2 × 1 PN, 1 × 3 PN, 9 × 2 PN | 10 | 0.71 | 5 × 2 PN | 0.5 |
| Average | 11.6 ± 5.1 | 7.67 ± 5.34 | 0.58 ± 0.29 | 7.13 ± 5.25 | 0.71 ± 0.32 | ||
| Total | 57.0 |
COC cumulus oocyte complex, ZP zona pellucida, GV germinal vesicle stage oocyte, MI meiosis I stage oocyte, MII meiosis II stage oocyte, deg degraded oocyte
aSuccessfully fertilized oocytes are the ones having two or three pronuclei
bFertilization rate is calculated dividing day 1 embryos showing two or three pronuclei (PN) with total amount of ICSI performed
cBlastocyst formation rate is calculated dividing day 5 embryos by successfully fertilized embryos