Fig. 2.

Effects of estrogen, progesterone, and human chorionic gonadotropin (hCG) on OVGP1 expression in human endometrial epithelial cells. a Expression of OVGP1 mRNA from Ishikawa cells. Lane 1 is 100 bp ladder, lane 2 cDNA from Ishikawa mRNA, and lane 3 no reverse transcriptase control. The bands for OVGP1 (172 bp) and GAPDH (170 bp) are shown by arrow marks. b Immunofluorescence for OVGP1 in Ishikawa cells. Red staining is for OVGP1, and blue is for the nuclei. Scale bar is 50 μm. A cell is digitally zoomed × 5 times and shown. c Western blotting for OVGP1 in protein isolated from Ishikawa cells. Lane 1 is negative control (incubated without primary antibody), and lane 2 is sample incubated with primary antibody. The approximate size of the bands is marked with an arrow. All the experiments were done at least three times. Ishikawa cells were treated with estrogen (E₂), progesterone (P₄), and hCG alone or in combinations (e–i) for varying time points, and level of OVGP1 mRNA was measured by real-time PCR. Y-axis is fold change as compared to untreated control at each time point (control value taken as 1). X-axes are time points in hours post-treatment. Values are mean ± SD for six replicates. * indicates mean value significant different (p < 0.05) as compared to respective control