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. 2018 Aug 10;8:11972. doi: 10.1038/s41598-018-30446-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects on tube formation in HUVECs treated with exosomes released from PK-45H cells. Tube formation assay was performed using the Cultrex In Vitro Angiogenesis Assay Tube Formation Kit (Cosmo Bio). A dynamin-dependent exosomal endocytosis promoted tube formation in HUVECs. Data are expressed as means ± standard deviations (each n = 3). Statistical significance (P ≤ 0.05) was examined with the Tukey–Kramer test for multiple comparisons.