Table 2.

Sequencing results comparing WGA and double-stranded DNA synthesis

Virus	Double-stranded DNA synthesis		Fold change in detection
	First strand → WGA	First strand → second strand
Total reads	342 470 258	239 327 708
EBV	3.73% (12 770 076)	0.44% (1 049 436)	0.12
RSV	0.00038% (1287)	0.0094% (22 579)	25
FeLV	0.00030% (1032)	0.030% (71 024)	100
Reo3	0.015% (52 114)	0.31% (752 834)	21

The introduction of any bias in the viral population after WGA and/or double-stranded DNA synthesis was assessed using high-throughput sequencing. Extracted nucleic acid was converted to double-stranded DNA by first carrying out first-strand synthesis. Following first-strand synthesis the two techniques were compared for conversion to double-stranded DNA