Table 3.

Comparison of the selected sample preparation procedure to extraction using the QIAamp^® MinElute^® Virus Spin kit followed by WGA

Virus	MinElute® + WGA (average of n = 3 ± standard deviation)	Optimized procedure (average of n = 4 ± standard deviation)	Fold change in detection
EBV	0.36 ± 0.15%	0.34% ± 0.10	0.94
RSV	0.0012 ± 0.0007%	0.0076% ± 0.0021	6.3
FeLV	0.0022 ± 0.0014%	0.036% ± 0.008	16
Reo3	0.000045 ± 0.000038%	0.38% ± 0.14	8400

The selected procedure, which involved the dual extraction strategy using both Invitrogen PureLink™ Virus RNA/DNA kit and the Wako DNA Extractor® kit with the RNase A digestion and the double-stranded DNA synthesis, was compared against the QIAGEN QIAamp® MinElute® Virus Spin kit followed by whole-genome amplification (WGA). Reported values are an average of at least three replicates with their standard deviation