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. 2018 Aug 10;8:11991. doi: 10.1038/s41598-018-30360-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescent staining images, analysis of cell and nuclei alignment on FPC and GPC. Representative immunofluorescence staining of sarcomeric α-actinin (red) and nuclei stained with DAPI (blue) on FPC (a) and GPC (c). Relative polar plots of the orientation angle of cells on FPC (c) and GPC (d) are also reported. In this polar plot the distance from the origin, R, is the elongation factor, each point is a single cell (81 cells for FPC and 85 cells for GPC) and the angle to the horizontal, θ, is the angle the long axis of the cell makes with the reference axis. In (e) is shown the modulus of the angle of nuclei alignment on uniformly covered and collagen-patterned polyacrylamide with **p < 0.01.