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. 2018 Aug 10;8:11951. doi: 10.1038/s41598-018-30356-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot analysis of rNDV expressing S1 protein of IBV. The expression of codon optimized S1 protein of IBV expressed from four individual rNDVs expressing four different expression cassettes of S1 protein were detected using Western blot in cell lysates (A) and cell supernatant (B) of infected DF-1 cells infected with rNDVs, using a chicken polyclonal anti IBV serum. The lanes 1–5 represent cell lysates of rNDV, rNDV/S1, rNDV/S1 + IBV-S-TM&CT, rNDV/S1(cs−)+NDV-F-TM&CT, rNDV/S1(cs+) + NDV-F-TM&CT, respectively. A ~130 kD band represent expression of S1 protein by rNDV/S1 + IBV-S-TM&CT, rNDV/S1(cs−) + NDV-F-TM&CT and rNDV/S1(cs+) + NDV-F-TM&CT in infected DF-1 cell lysate (A lanes 3–5) and rNDV/S1 in infected DF-1 cell supernatant (B-lane 2). The full-length gel is presented in Supplementary Figure S1.