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. 2018 Aug 10;8:11951. doi: 10.1038/s41598-018-30356-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Multicycle growth kinetics of the rNDV constructs in DF-1 cells. DF-1 cells were infected with each recombinant virus at a MOI of 0.1. Two hundred µl of supernatant from infected cells were collected and replaced with fresh DMEM including 10% normal allantoic fluid at 8 hours intervals. The titer of the virus in harvested samples were determined by TCID₅₀ assay in DF-1cell.