Figure 2.

Quantitative analysis of the capture and release of hCNTF on the immobilized surface of magnetic beads. (A) WB of BYL based CFPS of hCNTF, lanes: (1&15): molecular weight marker; (2–3): total protein before split-intein mediated capture & intein reaction; (4–5): the remnant of the liquid fraction after immobilisation; (6–7): release of hCNTF after 6 hours of light triggered release (365 nm; 12.5 mW); (8–14): 200, 50, 10, 0.5, 0.125 ng of purified His₆-hCNTF. (B) WB of HeLa cell lysate based CFPS of hCNTF, lanes: (1&13): molecular weight marker; (2–3): total protein before split-intein mediated capture & intein reaction; (4–5): the remnant of the liquid fraction after immobilisation; (6–12): 40, 20, 10, 2.5, and 1.3 ng of purified hCNTF. The uncropped images can be seen in Fig. S6.