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. 2018 Aug 10;8:11967. doi: 10.1038/s41598-018-30435-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transfer of biotin to hCNTF upon PTS. (A) Comparison of peptide 1 and peptides 5–7 (biotin-conjugated peptides). Lanes: (1) molecular weight marker; (2) Laemmli buffer; (3) HeLa lysate without DNA (negative control); (4) HeLa CFPS expressed hCNTF-NpuDnaE_ΔC16; (5) Peptide 1; (6–8) Peptide 5–7; (9–12): hCNTF-NpuDnaE_ΔC16 protein trans splicing with peptides 1, 5, 6, and 7 respectively. Inset: magnification of the hCNTF (−) and hCNTF-biotin bands (+). (B) Lanes: (1&10) molecular weight marker; (2) HeLa CFPS expressed hCNTF-NpuDnaE_ΔC16 (total protein); (3) HeLa CFPS expressed hCNTF-NpuDnaE_ΔC16 with peptide 1 (protein trans splicing reaction); (4) HeLa CFPS expressed hCNTF-NpuDnaE_ΔC16 with peptide 5 (protein trans splicing reaction); (5) HeLa CFPS expressed hCNTF-NpuDnaE_ΔC16 with peptide 1 (protein trans splicing reaction) and streptavidin; (6) HeLa CFPS expressed hCNTF-NpuDnaE_ΔC16 with peptide 5 (protein trans splicing reaction) and streptavidin; (7–9) Laemmli buffer; (*) Streptavidin bound to biotinylated hCNTF. The white dashed lines indicate the front of the bands. Uncropped images can be seen in Fig. S9.