Table 1.

Expression, capture, and light release efficiency of hCNTF with immobilization on the surface of magnetic beads.

CFPS system	Total amount hCNTF [mg/L]a	Amount hCNTF Captured beads [%]	Amount hCNTF Releasedb [%]
BYLc	20.0	84	>6.3e
HeLac	49.2	93	ND
HeLad	ND	ND	>19e

^aMilligram hCNTF per liter of CFPS matrix determined with hCNTF standard in the WBs; ^bThe percentages are based on the relative volumes of the bands of hCNTF released from the magnetic beads compared to the total synthesized hCNTF in the same WB gels; ^cImmobilized peptide followed by split-intein mediated capture, washing, and light triggered release; ^dsplit-intein mediated capture and protein trans splicing in solution followed by immobilization, washing, and light triggered release; ^ecorrected values are based on negative controls (Fig. S4). ND = Not Determined.