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. 2018 Aug 10;8:11996. doi: 10.1038/s41598-018-30425-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cumulative wheel running revolutions in the first (a) and second 24 h periods (b) after i.p. administration of PBS, Fb-hemoCD1, and hemoCD1. Each bar represents the mean ± SE (n = 3 mice per group). The mice were housed for two weeks under a 12 h light/dark (LD) cycle (lights on at 7:00 and light off at 19:00) until the day before the experiments. The mice were then housed under constant dark (DD) conditions during the observations. Asterisk denotes statistical significance (^*P < 0.05), as compared to the controls. The raw actograms for measuring the wheel running activity are shown in Fig. S4.