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. 2018 Aug 10;8:11996. doi: 10.1038/s41598-018-30425-6

Figure 7.

Figure 7

ROS-induced circadian rhythm disruption mediated by TNF-α. (a) Schematic representation of the clock system involving Rev-erbα. Similar to Per and Cry, BMAL:CLOCK(NPAS2) enhances transcription of Rev-erbα. Additionally, TNF-α, which inhibits Per and Cry, slightly induces Rev-erbα. REV proteins inhibits the expression of BMAL1. (b) Changes in the relative mRNA levels of Rev-erbα in the murine liver after i.p. administration of hemoCD1. The mice were housed for two weeks under a 12 h light/dark (LD) cycle (lights on at 7:00 and light off at 19:00) until the day before the experiments. The mice were then housed under constant dark (DD) conditions during the observations. PBS and Fb-hemoCD1 were similarly administered as controls. Each bar represents the mean ± SE (n = 3 mice per group). Asterisk denotes statistical significance (*P < 0.05, **P < 0.01), as compared to the controls. The components of Fig. 7a were drawn using ChemBioDraw.