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. 2018 Aug 10;8:11988. doi: 10.1038/s41598-018-30418-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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K⁺ depletion reduces the mADK activity in cells. HEK293 monolayers were subjected to hypotonic shock by 30 minutes followed by incubation in an isotonic K⁺ -free Buffer. After this, the cells received K⁺ -Buffer (0, 2.5 and 5 mM KCl) and the control cells received a DMEM medium. The cells were remained by1 hour under each evaluated condition. After the treatments, the cells were lysed and the kinase activity (pg e-AMP/ µg PTN) measured by HPLC quantification analysis. Statistical analysis: one-way ANOVA. Post hoc test: Bonferroni (*For control P < 0.05). (Error bars represent 6 SD of the mean).