Figure 3.

Gene expression patterns in duodenum and kidney of Leuven mice on different diets (n = 12/group) and of Tokyo mice on normal diet (n = 6/group). (A) Duodenal gene expression of CaT1 , calbindin-D_9K , and PMCA , assessed by qRT-PCR analysis, is calculated as a ratio to the HPRT RNA level and is expressed relatively to levels of WT mice on normal diet. (B) Calbindin-D_9K protein content in duodenum, measured by RIA, is expressed as % (mg/mg) of total duodenal protein. (C) Renal gene expression of ECaC , calbindin-D_9K , calbindin-D_28K , PMCA , and NCX , assessed by qRT-PCR analysis, is calculated as a ratio to the HPRT RNA level and expressed relatively to levels of WT mice on normal diet. *, P < 0.001, †, P < 0.01, and ‡, P < 0.05 vs. WT on normal diet.