Table 1.
Phenotypic characterization of Leuven mice raised on different diets and of Tokyo mice raised on a normal diet
| Leuven: low calcium diet
|
Leuven: normal diet
|
Leuven: rescue diet
|
Tokyo: normal diet
|
|||||
|---|---|---|---|---|---|---|---|---|
| WT (n = 12) | KO (n = 12) | WT (n = 12) | KO (n = 12) | WT (n = 12) | KO (n = 12) | WT (n = 6) | KO (n = 6) | |
| Femoral calcium (mg/g of dry weight) | 15.5 ± 0.7* | 11.5 ± 0.5*† | 20.6 ± 0.4 | 17.3 ± 0.5* | 20.0 ± 0.4 | 20.1 ± 0.5† | 19.2 ± 0.7 | 13.3 ± 0.6* |
| Serum calcium (mg/dl) | 7.4 ± 0.4* | 4.2 ± 0.1*† | 10.1 ± 0.3 | 7.3 ± 0.3* | 10.2 ± 0.2 | 10.1 ± 0.4† | 8.6 ± 0.2 | 6.0 ± 0.1* |
| Serum PTH (pg/ml) | 41 ± 14 | 345 ± 50*† | 17 ± 3 | 182 ± 34* | 16 ± 1 | 15 ± 1.1† | ||
| Serum 1.25(OH)2D3 (pg/ml) | 226 ± 28 | 1843 ± 261*† | 57 ± 7 | 3634 ± 268* | 19 ± 4 | 9 ± 1.9† | ||
| Kidney Cyp27B1 RNA level | 57 ± 8.9 | 144 ± 21* | 2 ± 0.3 | 276 ± 49* | 2 ± 0.6 | 12 ± 10† | 1 ± 0.2 | 177 ± 18* |
Cyp27B1 RNA levels, assessed by qRT-PCR, are expressed as a ratio to the HPRT RNA level.
*
, P < 0.001 vs. WT on normal diet;
†
, P < 0.001 vs. KO on normal diet.