Table 3.
Duodenal and renal ECaC and CaT1 gene expression in Leuven mice raised on different diets and in Tokyo mice raised on a normal diet
| Leuven: low calcium diet
|
Leuven: normal diet
|
Leuven: rescue diet
|
Tokyo: normal diet
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| WT (n = 12) | KO (n = 12) | WT (n = 12) | KO (n = 12) | WT (n = 12) | KO (n = 12) | WT (n = 6) | KO (n = 6) | ||
| Duodenum | ECaC | 8.2 ± 1.1* | 0.4 ± 0.1* | 4.4 ± 0.8 | 1.6 ± 0.4† | 0.9 ± 0.2* | 0.4 ± 0.2* | 12 ± 3 | 1.0 ± 0.4† |
| CaT1 | 18,061 ± 1158* | 40 ± 11* | 3,020 ± 552 | 283 ± 74* | 64 ± 15* | 18 ± 8* | 2,940 ± 636 | 97 ± 26† | |
| Kidney | ECaC | 2,608 ± 216 | 1,726 ± 124 | 2,927 ± 554 | 3,618 ± 826 | 2,627 ± 286 | 4,703 ± 1010 | 3,684 ± 572 | 4,484 ± 420 |
| CaT1 | 21 ± 2 | 27 ± 3 | 22 ± 4 | 30 ± 4 | 26 ± 4 | 45 ± 8* | 31 ± 6 | 64 ± 5† | |
ECaC and CaT1 RNA levels, assessed by qRT-PCR, are expressed as a ratio to the HPRT RNA level.
*
, P < 0.001 and
†
, P < 0.01 vs. WT on normal diet.