Figure 2.
Subclustering Reveals Presence of Rare Cell Types
(A) Expression pattern of R23E10-Gal4 highlighting the dFBs in the adult brain. Scale bar, 50 μm.
(B) Heatmap showing the mapping of FAC-sorted scRNA-seq on the Seurat clusters.
(C) t-SNE showing seven subclusters within cluster 61.
(D) Heatmap showing the mapping of FAC-sorted scRNA-seq on these subclusters.
(E) t-SNE showing five subclusters within the lamina cluster.
(F) Heatmap showing the mapping of the RNA-seq data of the lamina monopolar cells from Tan et al. (2015) on the lamina subclusters.
(G) t-SNE showing ICIM clustering of SMART-Seq2 data from Li et al. (2017). Clusters are colored by co-expression of selected genes.
(H) t-SNE showing ICIM subclustering of the OPN cluster. Clusters are colored by co-expression of selected genes.
(I) t-SNE showing the subclustering of dopaminergic neurons, revealing the presence of the PAM cluster by expression of Fer2.
(J) t-SNE showing the separation of octopaminergic and tyraminergic neurons in cluster 64 by expression of Tbh.
(K) Subclusters of neurons colored by co-expression of neuropeptides.
(L) Table comparing the number of cells present in our dataset with the estimated numbers from literature.