Figure S6.

Cross-Tissue and Cross-Samples Distribution of Interacting Cell Pairs for Selected Types of Cell-to-Cell Interactions, Related to Figure 5C

Interacting cell pairs are marked with white and cyan circles on the montage of IgD CD90 (B cell and T cell markers) staining of every sample of the dataset. Due to cell proximity in most cases cyan circles practically completely overlay white.

(A and B) Interaction of CD4 and CD8 T cells with ERTR stroma (change in odds ratio score correlates with change in interaction count).

(C–E) Interaction of granulocytes with CD4 T cells, dendritic cells and erythroblasts.

(F and G) Interaction of erythroblasts with stromal and B220(+) DN T cells. Interactions in (C-G) scored as increased in early MRL/lpr (−4,-5,-6) as compared to BALBc spleens (FDR of t test on normalized interaction counts between conditions < 0.05, difference in interaction counts > 0).

(H and I) Interactions of B220(+) DN T cells with CD8 T cells and stromal cells. These interactions scored as increased in intermediate and late MRL/lpr (−7,-8,-9) as compared to early MRL/lpr spleens (FDR of t test on normalized interaction counts between conditions < 0.05, difference in interaction counts > 0). More examples of cell type pairs with change in interactions across dataset can be found in associated online repository (see STAR Methods).